Hippocampal neurons (12DIV) cultured on coverslips were infected with HSV-tPA/GFP. Twenty hours after infection the coverslip was placed in an optical recording chamber and placed on an upright Leica microscope fitted with a heated stage. Images were taken with a 40X dry objective, and image analysis was performed using Metamorph from Universal Imaging Corporation. These images were deblurred using a contrained iterative deconvolution algoritm.

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Time-lapse movies of a 13DIV hippocampal neuron expressing tPA/GFP.